FACS sorting analysis of HCAEC cells transduced with endothelial specific endoAAV.
left: Only live (=low PI signal) cells were sorted for further analysis. right: 63% of the transduced cells showed a robust GFP signal.
This new endoAAV is now available to the research community.
Within the common size limits, the system can be used to package virtually ANY gene
or shRNA coding
sequence. Combine it with tissue specific promoters to further select for cell specificity or even use a TET-inducible promoter
to time the expression for exact and convincing data. By combining target expression with selection markers the system enables selection of transduction positive clones and a high yield of pure transduction positive cells.
Feel free to contact our experts at SIRION Biotech email@example.com
to receive an individually drafted quote for your research application.